Phi6 rna polymerase
WebApr 14, 2024 · RT-qPCR was carried out using the SARS-CoV-2 nucleocapsid gene N1 region as a target and a phi6 process control, both with single stranded RNA quantification standard dilutions, with the N1 region limit of detection of 0.4 gc/μL and limit of quantification of 4 gc/μL . These steps were carried out by the Environment Agency … WebJan 1, 2014 · The genetic information encoding for the phi6 polymerase complex (cDNA of the phi6 genome segment L) is introduced into the Pseudomonas host together with target specific DNA flanked by phage-specific packaging and replication signals ( see Fig. 5) [ 8 ]. Both constructs are cloned into plasmids downstream of the T7 promoter.
Phi6 rna polymerase
Did you know?
WebHere we propose a new general method for directly determining RNA sequence based on the use of the RNA-dependent RNA polymerase from bacteriophage phi6 and the chain terminators (RdRP sequencing). WebBacteriophage Φ6 contains three double-stranded RNA (dsRNA) genomic segments, L, M, and S. The RNA is located inside a core particle composed of multiple copies of a major …
WebDec 28, 2000 · Here we show that the 2 A resolution X-ray structure of the active polymerase subunit from the double-stranded RNA bacteriophage straight phi6 is highly similar to that of the polymerase of hepatitis C virus, providing an evolutionary link between double-stranded RNA viruses and flaviviruses. By crystal soaking and co-crystallization, we ... WebApr 1, 2003 · Consider the combination of structural and sequence comparison of the HCV, RHDV and Phi6 polymerases that maps a 40 amino acid insertion within region F of the …
Φ6 has been studied as a model to understand how segmented RNA viruses package their genomes, its structure has been studied by scientists interested in lipid-containing bacteriophages, and it has been used as a model organism to test evolutionary theory such as Muller's ratchet. Phage Φ6 has been … See more Φ6 (Phi 6) is the best-studied bacteriophage of the virus family Cystoviridae. It infects Pseudomonas bacteria (typically plant-pathogenic P. syringae). It has a three-part, segmented, double-stranded See more Φ6 typically attaches to the Type IV pilus of P. syringae with its attachment protein, P3. It is thought that the cell then retracts its pilus, pulling the … See more • Double-stranded RNA viruses See more 1. Detailed molecular description 2. Descriptions of tests of evolutionary theory by the Turner Lab 3. Descriptions of tests of evolutionary theory by the Burch Lab See more The genome of Φ6 codes for 12 proteins. P1 is a major capsid protein which is responsible of forming the skeleton of the polymerase complex. In the interior of the shell formed by P1 is the P2 viral replicase and transcriptase protein. The spikes binding to receptors … See more RNA-dependent RNA polymerases (RdRPs) are critical components in the life cycle of double-stranded RNA (dsRNA) viruses. However, it is not … See more WebMay 30, 2013 · Bacteriophage Φ6 contains three double-stranded RNA (dsRNA) genomic segments, L, M, and S. The RNA is located inside a core particle composed of multiple copies of a major structural protein, an RNA-dependent RNA polymerase, a hexameric NTPase, and an auxiliary protein.
WebThe genome of phage phi6 is composed of three dsRNA segments, the gene encoding P5 being located in the smaller genomic fragment [7]. The synthesis of an additional protein, P11, with an apparent molecular mass of about 25 kDa, is closely associated with the synthesis of P5, both proteins being similarly affected by mutations [8].
WebDec 28, 2000 · Here we show that the 2 A resolution X-ray structure of the active polymerase subunit from the double-stranded RNA bacteriophage straight phi6 is highly similar to that … nrl of hivWebOct 1, 2008 · The properties of the phi6 RNA-dependent RNA polymerase (RdRp) and its use in RNAi experiments are also introduced. Poly(A) polymerase, a template-independent … nrl offloads 2021WebDec 9, 2016 · A symmetry mismatch at the site of RNA packaging in the polymerase complex of dsRNA bacteriophage phi6. Journal of molecular biology 294, 357–372, doi: … nrl offsideWebCystovirus phi6 Click on organism name to get more information. Pseudomonas phage phi6 Disclaimer: The NCBI taxonomy database is not an authoritative source for nomenclature … nrl official tippingWebApr 11, 2024 · Hydrodynamic cavitation achieved enveloped virus phi6 reduction of up to 6.3 logs. ... RNA was considered degraded if there was a noticeable difference in the intensity of the bands of both targets after the selected treatment time compared with the corresponding bands at time 0. ... and oxidation of proteins in the nucleocapsid or … nrl on 9WebDuring maturation, single-stranded copies of these segments are packaged into preformed polymerase complex particles. Only phi6 RNA is packaged, and each particle contains only one copy of each segment. An in vitro packaging and replication assay has been developed for phi6, and the packaging signals (pac sites) have been mapped to the 5' ends ... nr logistics wallersteinWebOct 1, 2008 · The properties of the phi6 RNA-dependent RNA polymerase (RdRp) and its use in RNAi experiments are also introduced. Poly (A) polymerase, a template-independent polymerase, catalyzes the incorporation of AMP residues onto the free 3′-hydroxyl terminus of RNA, utilizing ATP as a precursor. night mare mount