Clontech pabai
WebAug 14, 2015 · zt624 pAbAi Clontech 酵母单杂交系统. zt625 pBiFC-bFosVC155 Addgene BiFC 蛋白相互作用检测系统. zt626 pBiFC-bFos(deltaZIP)VC155 Addgene BiFC 蛋白相互作用检测系统. zt627 mCerulean-N1 Addgene FRET 蛋白相互作用检测系统. zt628 mCerulean -C1 Addgene FRET 蛋白相互作用检测系统 WebJul 5, 2024 · Subsequently, we inserted the amplification product into the pMD19-T vector, and subcloned into the pAbAi vector upstream of the AbAr reporter gene ... (Clontech, PT1172-1). The yeast transformants were selected on SD/-Trp and transactivation activity was evaluated on SD/-Trp-His-Ura-Ade/ + X-α-gal plates. The yeast cells on the …
Clontech pabai
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WebNov 27, 2024 · Cloning of transcription factors (TFs) We employed the Yeast One-Hybrid assay (Y1H) (Clontech Y1H, Clontech, USA) to identify TFs that interact with the cloned promoters. Briefly, promoter sequences were cloned into pAbAi Vector (Clontech Y1H, Clontech, USA) to construct the bait vectors. To provide the “prey”, floral-derived cDNA … WebMar 3, 2024 · The RT300 sequence of QH34 was amplified and cloned into the pAbAi vector to form the pBait-AbAi plasmid, Y1H-RT300. The Y1H-RT300 plasmid was integrated into the genome of yeast ... (Clontech). For high stringency in the interaction tests to the RT300 sequence, ...
WebVector database is a digital collection of vector backbones assembled from publications and commercially available sources. This is a free resource for the scientific community that is compiled by Addgene. This page is informational only - this vector is NOT available from Addgene - please contact the manufacturer for further details. WebJul 25, 2024 · The pAbAi-ProAtFT and pGADT7-AaZFP3 vectors were cotransformed into the yeast Y1HGold strain using a Matchmaker One-Hybrid Library Construction and Screening Kit (Clontech). Cotransformed yeast cells were selected on SD/-Ura-Leu (a synthetic dropout medium lacking Ura and Leu) with or without AbA (0.6 mg L −1 ; Sigma …
WebFeb 28, 2024 · the pAbAi vector (Clontech). pAbAi constructs were then. separately integrated into the genome of the Y1HGold strain. by homologous recombination to generate six bait reporter. strains. WebApr 11, 2024 · According to the manufacturer’s proposal, the Matchmaker Gold Yeast One Hybrid System Kit (Clontech) was used for Y1H determination. The SlTCP10 and SlJA2 CDs were connected to pGADT7 to generate AD-TCP10 and AD-JA2 constructs. The SlJA2 promoter fragments were separately connected to the pAbAi vector to produce the …
WebMar 1, 2024 · Matchmaker Gold 单杂交文库筛选过程直接、快速、简单！. 第一步：将1-3个目标DNA序列（bait）重复串联后克隆到pAbAi中。. 第二步：将线性化的pBait-AbAi载体整合到Y1HGold酵母基因组中，获得携带bait的酵母菌株，. 并在SD/-Ura培养基上筛选。. 第三步：利用Insert Check PCR Mix ...
Web2 days ago · The amplified product was inserted into the pAbAi vector (Clontech, Palo Alto, CA, USA) to generate a proBoMY-pAbAi construct. The coding sequence of BoFAMA was cloned into the pGADT7 vector to generate pGADT7::BoFAMA recombinant construct. The primers are listed in Table S1. Subsequently, the constructs were transformed into yeast … truist bank post rd cumming gaWebJan 24, 2024 · The Y1H Gold strain is the compatible strain for pABAi vector provided by Clontech. Can we use Ym4271 (the strain for Y1H assay and has the same genotype of Y1H Gold strain) instead of Y1H Gold ... philip nicholas wilmotsWebTakara Bio—Home philipniceguy exoticshttp://www.labbase.net/Supply/SupplyItems-4229347.html truist bank po box 819WebDownload over 2,700 carefully annotated plasmid and sequence files including commonly used cloning vectors from all major suppliers. Open sequence files in SnapGene to view features, annotate, clone and use as a reference. Display enzyme sites, features, primers, ORFs, translations and more on plasmid maps or in detail on the sequence view. truist bank plant cityWebThe Y1H Gold strain is the compatible strain for pABAi vector provided by Clontech. Can we use Ym4271 (the strain for Y1H assay and has the same genotype of Y1H Gold … philip n gross lmft 23 sherman streetWebFeb 2, 2024 · The pAbAi vector was used to construct the bait vector, ... The coding region of FtbZIP85 was cloned into pGBKT7 (Clontech, Mountain View, CA, USA) as the prey. The bait and prey constructs were co-transformed into the yeast strain AH109, using the previously described method . The yeast strains were grown on a selection medium … philip nicholas burza